Directions: In the following text, some sentences have been removed. For Questions 41-45, choose the most suitable one from the list A-F to fit into each of the numbered blanks. There is one extra choice, which does not fit in any of the blanks. Mark your answers on the ANSWER SHEET. (10 points)
(41) After being touted in the early 1990s as “the medicine of the future,” gene therapy left an 18-year-old dead and three others with leukemia; in July it was tied to the death of a 36-year-old Illinois woman undergoing treatment for rheumatoid arthritis, although further investigation cleared her therapy of the blame. Gene therapy scientists, however, believe they can put the bad news behind them, thanks to a handful of recent developments and others just over the horizon.
(42) Researchers initially planned to treat hereditary disorders such as cystic fibrosis, in which normal gene products are deficient, by delivering functional copies of missing genes to cells that need them. Since then, scientists have expanded gene therapy’s possible applications to include “training” immune cells to hunt down cancer, building new blood vessels and making the immune system resistant to infection.
“We really don’t know the full dimension of what it can do,” says Arthur Nienhuis, a hematologist at St. Jude Children’s Research Hospital in Memphis and president of the American Society of Gene Therapy (ASGT). In addition to 12 cancer treatments and a heart treatment currently in large phase III clinical trials, there have been a handful of early-stage developments: in June doctors at New York—Presbyterian Hospital announced promising results from a phase I trial for Parkinson’s disease; a therapy that has restored sight to 70 congenitally blind dogs is being tested in humans at the University of Pennsylvania; and eight research groups are gearing up to test new HIV treatments. (43)
(44) It can target only those tissues that need it, “which is a major contrast with traditional pharmacotherapy, where you take a pill or receive an injection, and a very, very small portion of the injected or ingested drug actually arrives at the [correct] site,” says David Dichek, a cardiologist at the University of Washington. But ensuring that the gene reaches its target is no small feat. Trials can skirt this problem when targeted cells can be injected directly or easily removed- with the latter method, doctors can manipulate isolated cells in the lab and replace them in the patient later. But getting genes to inaccessible targets has been one of the field’s biggest hurdles.
(45) Viruses are good at delivering genetic payloads to cells; after all, that is what they do. If scientists strip viruses of their genetic material and replace it with therapeutic genes, viruses will deliver this payload to the cells instead. Different viruses do different things—some attack the liver, others nerves; some insert their DNA into the host genome, others do not—so physicians can choose those that best suit their purposes and further engineer them if need be. “There’s been a lot of effort to steer viruses to go specific places,” says Donald Kohn, an immunologist at the Keck School of Medicine of the University of Southern California and Children Hospital Los Angeles.
A. Even if gene therapy conquers these challenges, will it ever overcome its negative reputation?
B. What makes gene therapy so promising also makes it extremely challenging.
C. Most scientists use modified viruses as “vectors” to deliver gene therapy.
D. Although no gene therapies have yet been approved by the U.S. Food and Drug Administration, more than 800 trials are ongoing; China has approved two cancer treatments, but their efficacy remains unclear.
E. The past 15 years have been a roller coaster for gene therapy.
F. Gene therapy describes any treatment in which doctors insert new or modified genes into a person’s cells to treat or prevent disease.
